mcd99Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40study mht be useful for further studies of mCD99 function. Materials and Methods Cloning of expression vectors The previously cloned mCD99 (Park et al., 2005) was subcloned into